Evosep / Consumables / Columns, emitters and column heating
Consumables
Columns, emitters
and column heating
Standardized separation depends on matched parts. Every Evosep method has a specified column, every column has a matched emitter, and every Performance column has a defined temperature — which is what makes results transfer between runs, systems and laboratories.
Pre-mounted, finger-tight fittings
Zero dead volume
Four Pod variants
Eno = Performance columns only
Why matched parts
Standardization is the point
Evosep delivers reproducibility run-to-run, column-to-column and instrument-to-instrument — and the columns and emitters are a large part of how. Every analytical column ships with pre-mounted connection fittings that are finger-tight and free of dead volume, so the connection itself stops being a source of variability.
Each standard method has a recommended column, and each column has a specified operating temperature. Follow the pairing and you get the documented performance without method development. Deviate from it — a different column, an unheated Performance column — and the published metrics no longer apply.
Column lifetime. Partial elution from Evotip Pure keeps large, hydrophobic molecules off the analytical column, and the measured effect is a column that does not drift: across 1,056 consecutive injections on a single column, high-pressure pump stability held at 2.5 bar standard deviation, with no systematic rise in backpressure (AN-046A). Evosep states that Performance column lifetime extends to tens of thousands of injections.
Two lines, and only one of them runs on Eno. On Evosep One, Endurance runs the standard methods at ambient temperature — robust and reproducible everywhere, for everyone, every time. Performance delivers exceptional results but asks something in return: elevated column temperature control to manage system backpressure.
Evosep Eno
Which column for which method
Evosep Eno standard methods use Evosep Performance columns, all heated to 40 °C. The Whisper™ Zoom methods use IonOpticks columns at 50 °C.
Standard methods — Evosep Performance columns, 40 °C
| Method | Column | Part number | Temperature |
|---|---|---|---|
| 500 / 300 / 200 SPD | Performance column, 4 cm | EV1182 | 40 °C |
| 100 / 60 SPD | Performance column, 8 cm × 150 µm, 1.5 µm | EV1109 | 40 °C |
| 30 SPD | Performance column | EV1137 | 40 °C |
All three are compatible with Evosep Pod column ovens and EV1086 stainless steel emitters.
Whisper™ Zoom methods — IonOpticks columns, 50 °C
| Method | Column | Temperature |
|---|---|---|
| 120 / 80 SPD | IonOpticks Aurora Rapid, 5 cm × 75 µm C18 | 50 °C |
| 40 / 20 SPD | IonOpticks Aurora Elite, 15 cm × 75 µm C18 | 50 °C |
As used in application note AN-057A. See the Whisper Zoom data →
Evosep One
Matched columns for the standard and EVOSEP+ methods
Two lines, one table. Endurance columns run at ambient temperature; Performance columns require elevated temperature control.
This section applies to Evosep One only. The Endurance line is available to Evosep One customers and is not compatible with Evosep Eno.
| Method | Endurance line | Performance line |
|---|---|---|
| 300 SPD | EV1107 · 4 cm × 150 µm, 1.9 µm | EV1109 · 8 cm × 150 µm, 1.5 µm |
| 200 SPD | EV1107 · 4 cm × 150 µm, 1.9 µm | EV1109 · 8 cm × 150 µm, 1.5 µm |
| 100 SPD | EV1064 · 8 cm × 100 µm, 3 µm | — |
| 60 SPD | EV1064 · 8 cm × 100 µm, 3 µm | — |
| 30 SPD and extended | EV1106 · 15 cm × 150 µm, 1.9 µm | — |
| Whisper 40 SPD | EV1106 · 15 cm × 150 µm, 1.9 µm | EV1112* · 15 cm × 75 µm, 1.9 µm |
| Whisper 20 SPD | EV1106 · 15 cm × 150 µm, 1.9 µm | EV1112* · 15 cm × 75 µm, 1.9 µm |
* EV1112 is to be used only with the EV1111 emitter.
Endurance line — Evosep One only
Used in the Evosep One standard methods for robust, reproducible performance at ambient temperature — everywhere, for everyone, every time. Packed with ReproSil-Pur C18 beads by Dr Maisch. Not available for Evosep Eno.
Performance line — Evosep One and Evosep Eno
For exceptional results, with one requirement: elevated column temperature control to manage system backpressure. EV1109 and EV1115 use ReproSil Saphir C18, 1.5 µm; EV1112 and EV1118 use ReproSil-Pur C18, 1.9 µm.
Open-ended columns
Every column comes by default with fittings for Evosep emitters and spray adapters. When those are not appropriate for your setup, open-ended versions — marked OE — are available with a UHP union instead, so you can connect your own emitter.
EV1094 — Endurance OE, 8 cm × 100 µm, 3 µm
EV1113 — Endurance OE, 15 cm × 150 µm, 1.9 µm
EV1114 — Endurance OE, 4 cm × 150 µm, 1.9 µm
EV1115 — Performance OE, 8 cm × 150 µm, 1.5 µm
EV1118 — Performance OE, 15 cm × 75 µm, 1.9 µm
Emitters
Three emitters, integrated liquid junctions
Evosep emitters are made from either stainless steel or fused silica and come with integrated liquid junctions, for zero dead volume, finger-tight connections in Evosep source adapters. All are supplied in packages of two.
EV1086
Stainless steel · ID 30 µm
The standard choice, and the emitter specified for use with Evosep Pod column ovens and Evosep Eno Performance columns.
EV1087
Fused silica · ID 20 µm
Fused silica with an integrated liquid junction, for setups where a narrower bore is preferred.
EV1111
Fused silica · ID 10 µm
The narrowest option — and the required emitter for the EV1112 Performance column used by the Whisper 20 and 40 SPD methods.
Column heating
Evosep Pod™ — excellence just got easier
Evosep Pods are simple, standardized column ovens that connect your Evosep Eno to compatible mass spectrometers. Insert the column, close the Pod, start the analysis — the required temperature is applied across all Evosep Eno standard methods without any manual settings.
01
Seamless integration
Designed to work with Evosep Eno standard methods and Evosep Performance columns on your preferred MS interface, with a defined connection setup for each one.
02
Plug-and-play simplicity
A standardized, one-piece solution. No manual configuration, no temperature settings to remember or get wrong.
03
Optimized performance
Standardized method temperature keeps backpressure within its optimal working range and delivers consistent chromatography across all Evosep Eno standard methods.
04
Lab-to-lab transferability
Because the temperature is part of the method rather than a local decision, separations reproduce when a method moves to another laboratory.
Do you need one? Bruker CaptiveSpray and SCIEX OptiFlow already include excellent column oven solutions, so no Evosep Pod is required for those interfaces.
One Pod per mass spectrometer interface: Waters NanoLockSpray, Thermo EASY-Spray, Thermo Nanospray Flex and Agilent Nanospray.
Find your Pod
| MS interface | Evosep Pod | Part number |
|---|---|---|
| Thermo EASY-Spray | Evosep Pod for Thermo EasySpray | EV1187 |
| Thermo Nanospray Flex | Evosep Pod for Thermo Nanospray Flex | EV1194 |
| Waters NanoLockSpray | Evosep Pod for Waters NanoLockSpray | EV1189 |
| Agilent Nanospray | Evosep Pod for Agilent Nanospray | EV1191 |
| Bruker CaptiveSpray | Column oven included with the source — no Pod required | |
| SCIEX OptiFlow | Column oven included with the source — no Pod required | |
Every Pod is compatible with the EV1182, EV1109 and EV1137 Performance columns and the EV1086 stainless steel emitter. The Evosep Pod is intended to be used as General Laboratory Equipment (GLE).
Ordering
Complete part number reference
Columns — Evosep Eno (Performance columns only)
| P/N | Part | Description |
|---|---|---|
| EV1182 | Performance column, 4 cm | Used by the 200, 300 and 500 samples/day methods. Operated at 40 °C. |
| EV1109 | Performance column, 8 cm × 150 µm, 1.5 µm | Used by the 60 and 100 samples/day methods. ReproSil Saphir C18, 1.5 µm beads by Dr Maisch. |
| EV1137 | Performance column | Used by the 30 samples/day method. Operated at 40 °C. |
Columns — Evosep One
Endurance columns are available to Evosep One customers only.
| P/N | Part | Description |
|---|---|---|
| EV1064 | Endurance column, 8 cm × 100 µm, 3 µm | Used by the 60 and 100 samples/day methods. Pre-mounted connection fittings. ReproSil-Pur C18, 3 µm beads by Dr Maisch. |
| EV1094 | Endurance column OE, 8 cm × 100 µm, 3 µm | Open-ended version with UHP union. |
| EV1106 | Endurance column, 15 cm × 150 µm, 1.9 µm | Used by the 30 samples/day and extended methods. ReproSil-Pur C18, 1.9 µm. |
| EV1113 | Endurance column OE, 15 cm × 150 µm, 1.9 µm | Open-ended version with UHP union. |
| EV1107 | Endurance column, 4 cm × 150 µm, 1.9 µm | Used by the 200 and 300 samples/day methods. ReproSil-Pur C18, 1.9 µm. |
| EV1114 | Endurance column OE, 4 cm × 150 µm, 1.9 µm | Open-ended version with UHP union. |
| EV1109 | Performance column, 8 cm × 150 µm, 1.5 µm | Used by the 60 and 100 samples/day methods. ReproSil Saphir C18, 1.5 µm. |
| EV1115 | Performance column OE, 8 cm × 150 µm, 1.5 µm | Open-ended version with UHP union. |
| EV1112 | Performance column, 15 cm × 75 µm, 1.9 µm | Used by the Whisper 20 and 40 samples/day methods. Use only with the EV1111 emitter. |
| EV1118 | Performance column OE, 15 cm × 75 µm, 1.9 µm | Used by the nano-flow methods. Open-ended with UHP union. |
Emitters
| P/N | Emitter |
|---|---|
| EV1086 | Stainless steel, ID 30 µm — package of two |
| EV1087 | Fused silica, ID 20 µm — package of two |
| EV1111 | Fused silica, ID 10 µm — package of two |
Column ovens
| P/N | Evosep Pod |
|---|---|
| EV1187 | For Thermo EasySpray |
| EV1194 | For Thermo Nanospray Flex |
| EV1189 | For Waters NanoLockSpray |
| EV1191 | For Agilent Nanospray |
Product page
Evosep Eno™ — LC separation platform
The ten standard and Whisper Zoom methods, performance data, technical specifications and service coverage.
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