Evosep Proteomics / Evosep Lupo
Sample preparation system
Evosep Lupo™
A dedicated platform that executes standardized Evokits end to end — from protein lysate to loaded, ready-to-analyse Evotips — with roughly ten minutes of hands-on time per plate and a complete traceability record for every sample.
96 · 192 · 384 samples per run
Walk-away operation
Built-in QC
LIMS and CDS compatible
Evosep Lupo is for General Laboratory Use.
~10 minTotal hands-on time per plate — setup and output combined
<6 hRun time for a 96-sample Evokit Digest; <8 h for 384 samples
92%Protein overlap between plates run on two independent Lupo systems
FullTraceability — every step, reagent and consumable captured in the run record
Why a dedicated platform
Standardization you cannot drift away from
General-purpose liquid handlers give you flexibility — and with it, every opportunity for protocols, reagents and operators to diverge. Evosep Lupo takes the opposite approach.
Methods are preset on the instrument, exactly as on Evosep LC systems, and the platform runs Evokits only. Consumables, protocol and instrument are co-designed and released together, so performance is characterised as a system rather than assembled from parts. The result is a workflow that behaves the same way on Monday and on Friday, in your lab and in a collaborator’s.
No method development. No optimisation. Select the protocol associated with the Evokit, choose the loading percentage, and run.
Plug and play
Up and running immediately — no configuration effort or specialised training.
Designed exclusively for Evokits
Performance is fully optimised and standardized; no variability from mixing components.
Vision failsafe technology
Intelligent checks for consumables, plate positioning and workflow execution.
Barcode traceability
Data matrix codes on all components carry protocol identity and execution detail.
Fits your infrastructure
Compatible with LIMS and CDS, so the system does not create a new data silo.
How it works
Three touchpoints, one uninterrupted run
The starting point is a reduced and alkylated protein lysate — up to 1 µg in a maximum of 5 µl, with SDS at or below 0.5%. Everything downstream is handled by the system.
Setup · ~5 min hands-on
Load and confirm
- Insert the standardized Evokit — no preparation, modules stored under required conditions
- Guided setup on the instrument
- Integrated verification of position, orientation and expiry
- Direct sample list import
- Certificate of analysis available for the kit
Run · 0 min hands-on
Uninterrupted processing
- Magnetic bead-based (PAC) digestion, 4 h at ambient temperature
- Clean-up, peptide recovery and Evotip loading
- Process control and system suitability samples run alongside
- Real-time countdown and status
Output · ~5 min hands-on
Ready for the mass spectrometer
- Loaded Evotips, ready to analyse or store for up to 30 days
- MS-ready sequence list with controlled run order via Evotip positions
- Logfile with full traceability, exportable
Standardized kits
No preparation needed. Modules stored under required conditions.
Error-free setup
Built-in verification of correct position, orientation and expiry.
Built-in QC
Automated monitoring of kit and LC-MS performance. Supports additional study QCs.
Full traceability
Batch tracking. Easy access to certificate of analysis.
MS-ready export
Tailored .csv for your setup. Controlled run order via Evotip positions.
Performance
Transferable between plates, kits and instruments
From application note AN-058A. K562 lysates processed with Evokit Digest, 1×96 (EV3802) on Evosep Lupo; Evotips analysed with 500 SPD and 200 SPD methods on Evosep Eno, with discovery analysis on an Orbitrap Astral and targeted analysis on a Waters Xevo TQ-Absolute XR.
- 92.1%Overlap in identified proteins between two independent plates processed on two separate Evosep Lupo systems, across all identified proteins without missing-value filtering.
- ~99%Digestion efficiency from the ratio of fully cleaved to missed-cleavage peptides, consistently high across kits.
- 12.4–12.7%Protein group CV within and between Evokits and Evosep Lupo systems.
- <1 sRetention time variability across Evokits, injections and days, measured on system suitability reporter peptides.
- 10–15%Overall workflow CV across the complete preparation and analysis pipeline; LC-MS contribution alone stays below 5%.
Full methods, figures and conditions: application note AN-058A.
Challenged with real-world contaminants. A complex bone tissue matrix (1 µg starting material, 30% of digest loaded on Evotip, n = 8 per matrix) spiked with 4 M GdnHCl, 20% glycerol, 0.2 M glycine, 0.5 M NaCl, 2% NP40, 0.5% SDS, 0.5 M Tris, 1% Triton X-100, 2% Tween 20 and 4 M urea. Identifications stay stable and protein group CVs sit between 9.7% and 12.7%. Orbitrap Astral, 500 SPD. Source: AN-058A, Figure 4.
Quality control and traceability
Reproducibility verified inside every run
Two complementary controls ship inside the kit and execute as part of the routine workflow, so quality assessment adds no operational complexity.
Process Control Digest — 1 µg K562 in 0.5% SDS, processed twice per plate in positions A12 and B12, monitoring the full sample preparation workflow. The proposed LC-MS run order places them as injections 3 and 4.
System Suitability Control — reporter peptides that verify LC-MS performance over time by tracking retention time, peak shape, peak width and signal intensity. Chromatographic measures determine QC quality and can be assessed in real time.
- User-linked recordsEach action is linked to the user and the sample.
- Real-time traceabilityData is captured as you work.
- Single sourceOne sample list creates the LC-MS sequence, exported to avoid transcription errors.
- Fully coveredAll steps, reagents and consumables tracked, with data matrix codes on every component.
- Consistent and accessibleStandardized workflows with saved, exportable records.
Default run order of QC samples. Process control samples monitor the full sample preparation workflow; system suitability controls verify LC-MS performance over time.
The output view lists every experiment with kit, loading percentage and sample count, including QC samples — with report, copy and export actions. Sequence lists export as a tailored .csv for Thermo, Bruker, Waters, SCIEX and Agilent software.
Specifications
Operating envelope
Sample input (Evokit Digest)
| Sample volume | Maximum 5 µl |
| Protein input | Maximum 1 µg lysate — reduced, alkylated, DNA-free |
| SDS | Maximum 0.5% final concentration |
| Sample types | Biofluids, cultured cells, tissue lysates |
| Lower range | Linear with good quantification down to 8 ng lysate (30% of digest loaded). Not intended for high-sensitivity or single-cell work. |
| Normalisation | BCA assay recommended for protein input |
Instrument and run
| Throughput | 96, 192 or 384 samples per run |
| Plate handling | Up to 4 × 96-well plates processed in parallel |
| Run time | <6 h (96-sample kit) · <8 h (384-sample kit) |
| Digestion | 4 h, ambient temperature; efficiency stable down to 20 °C |
| Chemistry | Magnetic bead-based (PAC) digestion protocol |
| Endpoint | Loaded, ready-to-analyse Evotips (30-day storage window) |
| Control computer | Supplied with the instrument |
| Export | Tailored .csv sequence list for SCIEX, Waters, Thermo, Bruker and Agilent software |
Validated contaminant tolerance
Evokit Digest is optimised to work in the presence of common lysis buffer components at these concentrations. SDS is removed during the workflow and is not loaded onto the Evotip.
Triton X-100 · 1%SDS · 0.5%
GdnHCl · 4 MNaCl · 0.5 M
Urea · 4 MTris · 0.5 M
NP40 · 2%Tween 20 · 2%
Glycerol · 20%Glycine · 0.2 M
TEAB · 50 mM
Consumables
Evokits — everything the run needs, in one box
Evokits combine reagents, labware, Evotips and process controls with a predefined protocol, packaged for direct use in Evosep Lupo. Data matrix codes on all components carry protocol identity and execution detail, and each kit ships with a certificate of analysis.
Evokit Digest
- Standardized digestion for consistent, high-quality sample preparation
- Magnetic bead-based protocol for efficient clean-up and peptide recovery
- Broad sample compatibility across biological sample types
- Integrated process control monitors efficiency between batches
- Up to 384 samples per run
Evokit System Suitability for LC-MS
- Monitors LC-MS system suitability for consistent performance
- Enables longitudinal studies with reliable monitoring across batches
- Ideal for weekly maintenance and between projects
- Standardizes system suitability across instruments and labs
- Up to 96 samples per run
Ordering information
| Part number | Product | Description |
|---|---|---|
| EV3000 | Evosep Lupo | Sample preparation instrument for processing Evokits |
| EV3802 | Evokit Digest, 1×96 | Reagents, process control samples and labware for up to 96 samples onto Evotips |
| EV3803 | Evokit Digest, 1×384 | Reagents, process control samples and labware for up to 384 samples onto Evotips |
| EV3801 | Evokit SSC LC-MS, 1×96 | Reagents, system suitability control samples and labware for 96 SSC Evotips |
| EV3001 | Sample tip adapter | Holder for sample tips within Evosep Lupo — user replaceable |
| EV3002 | Evotip adapter | Holder for Evotips within Evosep Lupo — user replaceable |
| EV3003 | Evokit adapter | Holder for 4 °C and −20 °C Evokit components — user replaceable |
Bulk configurations (10×, 30× and 100×) are available for Evokit Digest and Evokit SSC LC-MS. Kits ship at room temperature and should be stored under the specified conditions; initial shelf life is 6 months. Contact us for pricing and availability.
Frequently asked
Evosep Lupo, in practice
Can I run fewer than 96 samples, or save part of a kit for later?
The complete Evokit is processed at once — once reagents are pierced and dissolved, stability cannot be controlled over longer periods. Fewer than 96 samples can be processed, but the instrument will still handle the full plate and consume reagents accordingly. We recommend pooling to prepare as many samples as possible per run.
Can I modify the methods or program my own protocols?
No. Methods are preset on the instrument, as with Evosep LC systems. The platform is standardized and works with Evokits only — this is what allows the performance of the complete workflow to be guaranteed.
Can Evokits be run on another liquid handler?
No. Evokits are not supported on other liquid handlers, and Evosep Lupo can only run Evokits manufactured by Evosep. Kits can be purchased without the instrument, but they are designed for automation and can only be processed on Evosep Lupo.
Can Evosep Lupo load Evotips prepared elsewhere?
No. The endpoint of every Evokit is a loaded, ready-to-analyse Evotip. The system cannot load samples prepared manually or on a different liquid handler.
Why automate if I don’t run large cohorts?
To reduce hands-on time and increase consistency for every sample. Automation delivers the same result each time and, with standardized Evokits, requires no optimisation — alongside built-in traceability, minimal required experience and seamless integration with Evosep LC.
Is the instrument temperature controlled during digestion?
Digestion runs at ambient temperature for 4 hours. Digestion efficiency is stable across temperatures and has been tested down to 20 °C.
Does it support a 384-well plate format?
Samples should be aliquoted into 4 × 96-well plates prior to preparation. The instrument processes up to four plates in parallel — 96, 192 or 384 samples per run.
Can different loading percentages be used across one plate?
No. One loading amount is used across a plate.
Can Evosep Lupo perform peptide quantification?
No, peptide quantification is not part of the workflow. We recommend a BCA assay to normalise protein input before the run.
What criteria determine whether a run should continue or pause?
Chromatographic measures are used to determine the quality of the QC samples, and this assessment can be made in real time.
Will PTM enrichment be supported?
The initial Evokit portfolio covers digestion and quality control, with a clear path toward application-specific kits. The ambition is to enable enrichment workflows as well.
How long will the instrument and kits be serviced?
Evosep will continue to service the instrument and Evokits. Performance Service Contracts are available to protect uptime and analysis productivity.
Go deeper
Documentation and related products
Application note · AN-058A
Standardized sample preparation at scale with Evokits and Evosep Lupo
Digestion efficiency, inter-robot reproducibility, protein group CV and retention time stability across plates, systems and ten challenging matrices.
Open in the literature room → Whitepaper · WP-002AEvosep Proteomics: a standardized ecosystem from sample to separation
How built-in controls, traceability and automation make reproducibility inherent to the system rather than dependent on operator skill.
Open in the literature room → OverviewEvosep Proteomics — the full workflow
How Evokit, Evosep Lupo, Evotip Pure and Evosep Eno combine into one controlled sample-to-separation system.
Explore the ecosystem →
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Explore Evosep Eno → ReferenceFull specifications and ordering
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See what Evosep Lupo does with your samples
Talk to an Evosep specialist about sample types, throughput and integrating Evosep Lupo into your existing LC-MS setup.