mRNA Purification Buffers
High-quality buffers for RNA purification
Consistent, high-quality buffers are of paramount importance for RNA purification to ensure their efficacy for downstream application and therapeutic use. Separation and purification of nucleic acids is most commonly performed using ion-paired reversed phase chromatography. This technique provides ionic interaction between the nucleic acid and the stationary phase via its hydrophobic moiety.
Hexylammonium Acetate Solution – HAA 2.0 M
Case of 6 x 200 mL bottles – Hexylammonium Acetate Solution – HAA 2.0 M
Hexylammonium Acetate Solution is a superior ion-pairing reagent from ADS Biotec developed specifically for reversed phase HPLC separations of DNA and RNA. The solution is manufactured for optimum performance in HPLC reversed phase separations whether with double-stranded, partially denatured or fully denatured nucleic acids. The material meets strict quality standards produced within an ISO-9001 certified quality system. HPLC buffers made using ADS Biotec HAA provide superior (low level) baseline in the chromatographic analysis of nucleic acids.
Features & Benefits
- Manufactured with the purest chemicals in a metal-free and clean environment
-Individual 200 mL bottles are convenient for preparing 2 liters of buffer for RP-HPLC chromatographic separations.
- Clean baseline and long shelf-life
- Highly reproducible retention times from batch to batch
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
Hexylammonium Acetate Buffer B – 0.1 M HAA in 50% Acetonitrile
Case of 4 x 2.5 liter bottles – Hexylammonium Acetate Buffer B
Hexylammonium Acetate Buffer B was specifically developed to provide highly reproducible retention times with HPLC System instrumentation. These Optimized buffers have a longer shelf life than buffers prepared from concentrate. They allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems. ADS Biotec HPLC buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically lead to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. ADS Biotec Optimized buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Features & Benefits
- Measurements are done gravimetrically to ensure correct concentrations of n-hexylamine hexylammonium acetate (HAA) and acetonitrile
- Manufactured with the purest chemicals in a metal-free and clean environment
-Buffer bottles are conveniently shaped and sized allowing the neck to fit most HPLC system caps
- Clean baseline and long shelf-life
- Highly reproducible retention times from batch to batch
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
- Minimizes operator exposure to acetonitrile
- Decreases evaporation of acetonitrile — a common cause of batch-to-batch variation in laboratory made and other purchased buffers
Hexylammonium Acetate Buffer A – 0.1 M HAA in 10% Acetonitrile
Case of 4 x 2.5 liter bottles – Hexylammonium Acetate Buffer A
The Optimized buffers were specifically developed to provide highly reproducible retention times with HPLC System instrumentation. Optimized buffers have a longer shelf life than buffers prepared from concentrate. They will allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems. Optimized buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically leads to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. Optimized Buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Features & Benefits
- Measurements are done gravimetrically to ensure correct concentrations of n-hexylamine hexylammonium acetate (HAA) and acetonitrile
- Manufactured with the purest chemicals in a metal-free and clean environment
-Buffer bottles are conveniently shaped and sized allowing the neck to fit most HPLC system caps
- Clean baseline and long shelf-life
- Highly reproducible retention times from batch to batch
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
- Minimizes operator exposure to acetonitrile
- Decreases evaporation of acetonitrile — a common cause of batch-to-batch variation in laboratory made and other purchased buffers
Triethylammonium Acetate Buffer B – 0.1 M TEAA in 25% Acetonitrile
Case of 4 x 2.5 liter bottles – Triethylammonium Acetate Buffer B
Triethylammonium Acetate Buffer B was specifically developed to provide highly reproducible retention times with HPLC System instrumentation. These Optimized buffers have a longer shelf life than buffers prepared from concentrate. They will allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems. ADS Biotec HPLC buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically leads to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. ADS Biotec Optimized buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Features & Benefits
- Measurements are done gravimetrically to ensure correct concentrations of TEAA and acetonitrile
- Manufactured with the purest chemicals in a metal-free and clean environment
-Buffer bottles are conveniently shaped and sized allowing the neck to fit most HPLC system caps
- Clean baseline and long shelf-life
- Highly reproducible retention times from batch to batch
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
- Minimizes operator exposure to acetonitrile
- Decreases evaporation of acetonitrile — a common cause of batch-to-batch variation in laboratory made and other purchased buffers
Triethylammonium Acetate Buffer A – 0.1 M TEAA in Water
Case of 4 x 2.5 liter bottles – Triethylammonium Acetate Buffer A
Triethylammonium Acetate Buffer A was specifically developed to provide highly reproducible retention times with HPLC systems. These Optimized buffers have a longer shelf life than buffers prepared from concentrate. They allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems. ADS Biotec HPLC buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically lead to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. ADS Biotec Optimized buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Features & Benefits
- Measurements are done gravimetrically to ensure correct concentrations of triethylammonium acetate (TEAA) and acetonitrile
- Manufactured with the purest chemicals in a metal-free and clean environment
-Buffer bottles are conveniently shaped and sized allowing the neck to fit most HPLC system caps
- Clean baseline and long shelf-life
- Highly reproducible retention times from batch to batch
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
- Minimizes operator exposure to acetonitrile
- Decreases evaporation of acetonitrile — a common cause of batch-to-batch variation in laboratory made and other purchased buffers
Triethylammonium Acetate Solution (TEAA) 2.0 M
Case of 6 x 200 mL bottles – Triethylammonium Acetate Solution 2.0 M
Triethylammonium Acetate Solution is a superior ion-pairing reagent from ADS Biotec developed specifically for reversed phase HPLC separations of DNA and RNA. The solution is manufactured for optimum performance in HPLC reversed phase separations whether with double-stranded, partially denatured or fully denatured nucleic acids. The material meets strict quality standards produced within an ISO-9001 certified quality system. HPLC buffers made using ADS Biotec TEAA provide superior (low level) baseline in the chromatographic analysis of nucleic acids.
Features & Benefits
- Manufactured with the purest chemicals in a metal-free and clean environment
-Individual 200 mL bottles are convenient for preparing 2 liters of buffer for RP-HPLC chromatographic separations
- Clean baseline and long shelf-life
HPLC Column Wash Solution D – Case
4 x 2.5 liter bottles HPLC Column Wash Solution D
Optimized Buffers were specifically developed to provide highly reproducible retention times with HPLC System Instrumentation. Optimized Buffers have a longer shelf life than buffers prepared from concentrate. They will allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems. Optimized Buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically leads to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. Optimized Buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
Features & Benefits
- Manufactured with the purest chemicals in a metal-free and clean environment
- Measurements are done gravimetrically to ensure correct concentrations of TEAA and acetonitrile
- Bottles are conveniently shaped and sized allowing the neck of the Optimized Buffer bottles to fit exactly into the HPLC system cap
-Highly reproducible retention times from batch to batch
- Clean baseline and long shelf-life
- Eliminates buffer transfer — just open the bottle and connect it to the HPLC system
- Minimizes operator exposure to acetonitrile
- Decreases evaporation of acetonitrile — a common cause of batch-to-batch variation in laboratory made and other purchased buffers
