Fintiede Solutions | Evosep
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Integrated Purification and Separation 

 The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.

Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.

Built For Standardization

The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.


Illustrated below using the 100 samples per day method and the standard column (EV-1064)

Failsafe Sample Loading With Evotips


Excellent Sample Stability 

  • Peptides are stable when immobilised on the tip (No cooled autosampler needed)
  • Loaded Evotips can be stored ready to inject
  • Peptides are stable for multiple days at room temperature 
  • Very robust proteome stability when stored cold up to 35 days at various loads

Three Functions Of A Single Evotip


Desalting Device

  • Sample loading immobilises peptides on the tip
  • Salts are washed from the tip

Sample Container

  • Peptides stay on the tip 
  • Tips are transferred to the Evosep One

Disposable Trap Column

  • Peptides are eluted into the Evosep One
  • Hydrophobic material remains in the tip
  • Tip is disposed off
Untitled design

How Can We Help?

If you require any further information on any of the Evosep products, Please give us a call on +44 (0) 7977 926 418 or drop us an email at​