The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
Built For Standardization
The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.
Illustrated below using the 100 samples per day method and the standard column (EV-1064)
Failsafe Sample Loading With Evotips
Excellent Sample Stability
Peptides are stable when immobilised on the tip (No cooled autosampler needed)
Loaded Evotips can be stored ready to inject
Peptides are stable for multiple days at room temperature
Very robust proteome stability when stored cold up to 35 days at various loads
Three Functions Of A Single Evotip
Desalting Device
Sample loading immobilises peptides on the tip
Salts are washed from the tip
Sample Container
Peptides stay on the tip
Tips are transferred to the Evosep One
Disposable Trap Column
Peptides are eluted into the Evosep One
Hydrophobic material remains in the tip
Tip is disposed off
How Can We Help?
If you require any further information on any of the Evosep products, Please give us a call on +44 (0) 7977 926 418 or drop us an email at info@fintiedesolutions.com