The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.
The speed of state-of-the-art mass spectrometers combined with the low overhead time and robustness on the Evosep One is a perfect match for high utilization. It is therefore needed to carefully review the balance of proteome depth versus high throughput before starting a series of experiments.
The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.
Illustrated below using the 100 samples per day method and the standard column (EV-1064)
Our five standard methods cover a broad range of applications. Explore more and find your favorite.
30 SPD: The longest method with the best overall proteome coverage. Download app note
60 SPD: The most popular method with an excellent balance of throughput and depth. Download app note
100 SPD: A great “in-between” method. Download app note
200 SPD: Excellent sequencing speed with most peptides per minute among our standard methods. Download app note
300 SPD: The highest peak capacity per minute with our fastest method for ultra-high throughput analysis. Download app note
The performance column is available for the 60 and 100 samples per day methods and is packed with 1.5 μM C18 beads. This reduces the peak widths with 2 seconds at FWHM and leads to an increase in peak capacity of 60%. Consequently, the proteome coverage is enhanced, and the column provides a robust and competitive commercial option – on the same level as home-pulled and packed options, but with better reproducibility and life-time. The boost in sensitivity is particularly noticeable for lower loads, where peptide and protein coverage is improved with 100%.