Fintiede Solutions | Bionavis
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About Bionavis

As the experts in Surface Plasmon Resonance, Bionavis aim to assist you on your way to absolute and high-quality results in life sciences, biosensors and material sciences. Here, you’ll find a brief introduction to their MP-SPR technology, applications and products.

LIFE SCIENCES 1

Life sciences

Drug development (Drug formulation, Controlled drug release, Drug-cell interaction, Nanoparticle targeting, Uptake mechanisms)

Biophysics (Lipid interactions)

Process optimization and control (Production quality, Cell growth matrix)

BIOSENSORS 1

Biosensors

Assay development (Agriculture, Food and feed safety, Environmental, Clinical, Border and Process control).

Development of point-of-care biosensors (EC, SERS, ELISA, fluorescence, printed biosensors, nanoparticles)

MATERIAL SCIENCES 1

Material sciences

New materials and coatings (Antimicrobial, Antireflective surfaces, Filter membranes, Contact lenses, Implants, Graphene, Carbon nanotubes, Green materials, Plasmonic materials,..)

Process optimization of thin film manufacturing (Display technologies, Solar cells, Batteries, ..)

Why choose Bionavis? (read more)

LIFE SCIENCES: Why MP-SPR?

• Unparalleled range: Optimize targeting from small molecule weight drugs up to nanoparticles and viruses
• Beyond affinity: Evaluate affinity as well as kinetics of the molecular binding events to provide decision tools for further selection
• Understanding: Understand better your molecules with kinetics and dynamic conformation change information from MP-SPR
• PureKinetics™: Bulk effect measured in-line ensuring premium quality data even with small molecules, lipids or biomaterials.
• Measurements from first selection of molecules up to uptake mechanisms: Quantify drug-material, drug-target, drug-lipid membranes and even drug-living cell monolayer interactions. Prove internalization label-free!
• No oil, no costly prism sensors: MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and also to further analyze with other methods such as microscopy.

 

The Firsts of MP-SPR:


• The only SPR to provide absolute measurement of interaction kinetics as well as layer structural changes.
• First SPR instrument to distinguish trans- and para-cellular drug/nanoparticle transport on cell monolayer in real-time and label-free!
• The first SPR with an in-line bulk effect measurement (PureKinetics™)

BIOSENSORS: Why MP-SPR?

• No costly assay transfer: develop your sensor directly on your sensing material: metals for electrochemical (EC) and SPR detection, plastics for ELISA, cellulose for printed biosensors, metal nanoparticles for SERS, glass for classical chemistry, magnetic nanoparticles, bacteria, living cells and more
• Any assay type: using direct binding, competitive assays, antibodies, fragments, DNA, molecularly imprinted polymers (MIPs), aptamers, nanoparticles, cells
• Any sample: Works with both gas and liquid samples, purified or crude, from small organic molecules up to metallic nanoparticles
• Easy validation: In-situ MP-SPR with electrochemistry, fluorescence, or other optical detection methods. Ex-situ: Microscopy.
• Beyond assay: Optimize also sample interaction with the disposable cartridge material to ensure binding only in the sensing area and not in the microfluidic channels.
• Understanding: Not just measurements, but unparalleled understanding of your assay kinetics and structural changes.

 

The Firsts of MP-SPR:


• The only commercial SPR providing absolute measurement!
• The widest range of sensing substrate materials in the market!
• The first SPR with an in-line bulk effect measurement (PureKinetics™)
• The first SPR to determine thickness and refractive index simultaneously (LayerSolver™)

MATERIAL SCIENCES: Why MP-SPR?

• Material freedom: Characterize nanolayers of metals, ceramics, polymers, organic materials and even nanocellulose or graphene
• Nanolayers from Å to µm: Measure and quantify materials from the single layer up to micron-thick multilayers
• Nanoparticles: Optimize nanoparticles and their adsorption kinetics
• LayerSolver™: Quantify both key parameters – thickness and refractive index – simultaneously
• Separate the best from better: The most sensitive measurement for kinetics on surfaces – real-time kinetics of adsorption, swelling, release.
• Minimum sample preparation: No freezing, no cutting, just apply your thin film to our substrate.
• From dry to wet state with same configuration
• Easy validation: In-situ MP-SPR with electrochemistry, fluorescence, or other. Ex-situ: Thanks to oil-free operation, same sample can be measured with AFM or other techniques. In-silico: Results can be confirmed also analytically.
• In-situ and Ex-situ modification of sensors: Our wide range of substrates can be modified with almost any thin film deposition technique including self-assembly, Langmuir-Blodgett (LB), Chemical Vapour Deposition (CVD), Evaporation, Atomic Layer Deposition (ALD), Molecular imprinting, Spin coating, Dip coating and others.

 

The Firsts of MP-SPR:


• First commercial SPR for material sciences
• The most sensitive instrument to study biochemical properties of functional coatings.
• The only instrument to measure alternating nanolaminate thickness from 5Å, optical density and its barrier functionality.
• The first SPR to determine thickness and refractive index simultaneously (LayerSolver™)

Bionavis technology (read more)

Multi-Parametric Surface Plasmon Resonance (MP-SPR)

Originally, surface plasmon resonance (traditional SPR) instruments have been developed for molecular interactions only. The technology has been used by pharma for more than 20 years with gold and dextran surfaces. Bionavis have pushed the limits of the technology to enable measurements on a number of other surfaces including polymers, graphene, nanoparticles and living cells.

 

More importantly, MP-SPR enables novel functions:

 

  • Measurements of molecular interactions with PureKinetics™
  • Measurements of layer properties with LayerSolver™

Measurements

Layer Properties

 

Refractive index
Thickness
Extinction coefficient
Density
Surface coverage
Swelling
Optical dispersion

Molecular Interactions

 

Kinetics
Affinity
Concentration
Adsorption/Absorption
Desorption
Adhesion
Electrochemistry

Substrates

MP-SPR expands substrates from traditional layers of gold, dextrans and other thin polymers to cellulose, PS, PET, PMMA, SiO2, TiO2, Al2O3, ITO, graphene, CNT, Pt, Pd and other.

 

While traditional SPR enables measurement of layers <150 nm thick, MP-SPR goes beyond this limit up to microns, enabling also work on living cell monolayers.

Theory of MP-SPR

An incident beam of p-polarized light strikes an electrically conducting gold layer at the interface of a glass sensor with high RI (Refractive Index) and an external medium/sample with low RI. At a given angle, the excitation of surface plasmons takes place resulting in a reduced intensity of the reflected light, which can be seen as a dip in the SPR curve.

 

Traditional SPR (such as focused beam BIA analysis, SPREETA, imaging SPR) measures only peak minimum at a single wavelength limiting its functionality and applicability. MP-SPR acquires whole SPR curve at multiple wavelengths providing high quality measurements of surface interactions as well as layer properties and enabling PureKinetics™ and LayerSolver™.

Traditional SPR measures only peak minimum at a single wavelength limiting its functionality and applicability.

MP-SPR acquires whole SPR curve at multiple wavelengths providing high quality measurements of surface interactions as well as layer properties.

About MP-SPR Navi™

The first instrument was installed in 2008 and is still running. The MP-SPR technology was introduced first in 2011, which has enabled a tremendous jump in the capabilities of the instruments as compared to traditional SPR.

 

Bionavis’s instruments are used by academia and industry in more than 25 countries around the world including USA, Japan, China, Finland, Turkey and Argentina. The newest generation of Bionavis instruments were released in 2015 introducing four main products of MP-SPR Navi™ family:

 

  • MP-SPR Navi™ 200
  • MP-SPR Navi™ 210A
  • MP-SPR Navi™ 220A
  • MP-SPR Navi™ 420A

 

Previously, Bionavis products included also SPR Navi™ 200, 210A and 220A as well as KSV SPR 200.

All instruments are designed and manufactured in Finland.

Bionavis products

Bionavis applications