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nano-Electrospray for Proteomics

The Sharp Singularity Emitters

 

Tolerances of the microscopic scale are required to ascertain stable and reproducible signals.

 

Fossiliontech’s unique micro-machining process produces:

  • Extremely Sharp Emitters
  • Constant Inner Diameter (down to 10 μm ID)
  • Very Tight Tolerances 

 

Fossiliontech ensure quality control reports and complete traceability with every emitter

The minimum wetting contact angle an emitter can accommodate is limited by its angle.

 

Sharper emitters can accommodate wider wetting angles. The electrostatic fields tangent to the surface of the emitter keeps the meniscus centered and aiming straight by compensating for the effect of the surface tension, which pushes the liquid to climb backwards.

 

Sharper emitters produce more stable sprays because the tangent component of the electrostatic field is stronger. Sharper emitters can accommodate a wider range of local electric fields (voltages), surface tensions and wetting contact angles.

The size of the meniscus is defined by the OD at the tip. Smaller meniscus lead to lower evaporation rates, better ionization efficiencies, and better detection limits in proteomic workflows. They require lower voltages and delay the onset of discharges, leading to more stable sprays. On the other hand, small ID are more prone to clogging, and age more rapidly. 

 

The ideal emitter diameter is the result of a balance: The largest possible ID and the smallest possible tip OD. 

 

The choice of the ID of the emitters is a trade-off decision, between performance and robustness.

– Pulled capillaries form extremely small tip IDs, but they can be prone to clogging because the inner diameter decreases along the capillary length.


– Micro-machined capillaries can be more repeatable and robust because they can reach very tight geometric tolerances with a constant inner diameter, less prone to clogging. But achieving very sharp edges is more difficult because more material needs to be removed.


– FIT’s unique micro-machining process produces very sharp emitters (7.5º semi-angle) with tight geometric tolerances and a constant ID. Thus combining the advantages of pulled and normal tapered emitters.

Emitter Sizes and Compatibility

Fossiliontech recommend specifying the right length for your application so that you do not need to cleave the emitter. The Sharp Singularity emitters are straight cut and polished at the back to ensure they sit properly in their fitting. For special requirements, contact us and we will be happy to assist you to.

Simple Link - UNO

Column to emitter link made simple:

Provides voltage to the electrospray and connects the column and emitter with an accessible Zero Dead Volume fitting

 

Ergonomic tool-free operation, reduces complexity and chances of error.

 

Comes in two versions: SIMPLE LINK UNO-16 for IDEX™ (1/16 OD sleeve, 13 nl swept volume) and SIMPLE LINK UNO-32 for VICI™ (1/32 sleeve, 1 nl swept volume) fittings.

 

Compatible with Nano Flex™

 

  • Recommended emitters: Sharp Singularity 7.5cm long

Simple Link UNO - 1/32"

Simple Link UNO - 1/16"

How Can We Help?

If you require any further information on any of the FossilIonTech products, Please give us a call on +44 (0) 7977 926 418 or drop us an email at info@fintiedesolutions.com